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Sperm Retrieval & Analysis

Sperm Retrieval & Analysis at Rotunda Hygeia

At Rotunda Hygeia, we offer advanced techniques for sperm retrieval and analysis — essential steps in diagnosing and treating male infertility, and for use in IVF or ICSI (Intracytoplasmic Sperm Injection) procedures.

Our expert andrology team ensures precise testing, compassionate care, and the best possible outcomes.

What is Sperm Retrieval?

Sperm retrieval is a procedure to collect sperm directly from the male reproductive system when sperm are absent in the ejaculate (a condition called azoospermia).

This is often needed when:

  • There is a blockage in the reproductive tract (obstructive azoospermia).
  • The testicles produce sperm, but none appear in the semen (non-obstructive azoospermia).
  • The man is unable to ejaculate due to certain medical or neurological conditions.

Sperm Retrieval Techniques

At Rotunda Hygeia, we use the most appropriate technique based on your condition:

1. TESA (Testicular Sperm Aspiration)

  • A fine needle is inserted into the testicle to aspirate sperm.
  • Simple and minimally invasive, usually done under local anesthesia.

2. PESA (Percutaneous Epididymal Sperm Aspiration)

  • Sperm are aspirated from the epididymis (where sperm are stored), using a thin needle.

3. Micro-TESE (Microsurgical Testicular Sperm Extraction)

  • A more advanced technique using a microscope to identify areas of sperm production in the testicle.
  • Ideal for men with very low or patchy sperm production.

4. Surgical Sperm Retrieval

  • In some cases, a minor surgical biopsy may be performed to extract sperm.

What Happens After Sperm Retrieval?

The sperm collected are examined and prepared in our state-of-the-art andrology lab:

  • If enough healthy sperm are retrieved, they can be used immediately for ICSI.
  • Alternatively, sperm can be frozen (cryopreserved) for future use.

What is Sperm Analysis?

Semen analysis, or sperm analysis, is the most basic and crucial test to assess male fertility.
It measures the quantity and quality of sperm in the ejaculate, checking for:
✅ Sperm count (concentration)
✅ Motility (movement)
✅ Morphology (shape and structure)
✅ Semen volume and viscosity
✅ Presence of infection or abnormal cells

Advanced Sperm Tests (if required)

In some cases, additional tests may be recommended, such as:

  • Sperm DNA Fragmentation Index (DFI) – to assess sperm DNA quality.
  • Reactive Oxygen Species (ROS) – to detect oxidative stress.
  • Chromatin structure assays.

These advanced diagnostics help in understanding the underlying causes of infertility and tailoring the best treatment plan.

Why Choose Rotunda Hygeia?

  • Expertise in all forms of sperm retrieval and analysis.
  • High-precision, modern andrology laboratory.
  • Advanced techniques to optimize sperm quality for IVF/ICSI.
  • Comprehensive care for male infertility in a discreet, supportive environment.

Book Your Appointment

If you’re struggling with infertility or require sperm retrieval for IVF, let the specialists at Rotunda Hygeia help you on your path to parenthood.


📞 Contact us today to schedule your consultation and take the first step.